Basic Matherial
|
Gene |
Insert |
Vector |
GenBank Access |
|
H1 (Mytilus edulis) Drabent et al. (1999) J. Mol. Evol. 49:645-655 |
3.5 Kb (Sac I) |
pUC-19 |
AJ224069 |
|
H2B (Holothuria tubulosa) Drabent et al. (1995) DNA Sequence 6:41-45 |
1.8 Kb (Hind III) |
pBSK(+) |
Z46225 |
|
H4 (Holothuria tubulosa) Drabent et al. (1995) DNA Sequence 6:41-45 |
1.5 Kb (Sac I / Pst I) |
pUC-19 |
Z46226 |
Image: H1, H2B and H4 clones from Mytilus edulis and Holothuria tubulosa.
Class Bivalvia
Subclass Pteridomorphia
Family Mytilidae
|
Species |
M.galloprovincialis |
M.californianus |
P. canaliculus |
M.chilensis |
|
|
Population |
Balcobo(B) |
Point no Point(Ca) |
Nueva Zelanda(NZ) |
Puerto Aguirre(PA) |
|
|
Species |
M.trossulus |
M.edulis |
|
Population |
Esquimalt Lagoon(T) |
Yerseke(Y) |
Subclass Heterodonta
Family Solenidae
|
Species |
E.ensis |
E.siliqua |
S.marginatus |
|
Population |
Means(EEM) |
Finisterre(EEF) |
Cambados(SMC) |
Familiy Veneridae
|
Species |
V.decussatus |
V.rhomboides |
V.pullastra |
R.philippinarum |
|
Population |
Cambados(VD) |
(VR) |
Carril(VP) |
(RP) |
|
Gene |
Template Sequence (GenBank Access) |
Software |
|
H1 |
Mytilus edulis (AJ224069) |
Oligo (Nucl.Acids Res. 17:8543-51) |
|
H2A |
Psammechinus lividus (Y09062/M25281)Psammechinus miliaris (V01141) Strongylocentrotus purpuratus (V01357) |
Oligo (Nucl.Acids Res. 17:8543-51) ClustalX (Nucl.Acids Res. 24:4876-82) GeneDoc 2.3.000 (Nicholas&Nicholas, 1997) |
|
H2B |
Holothuria tubulosa (Z46225) |
Oligo (Nucl.Acids Res. 17:8543-51) |
|
H3 |
Strongylocentrotus droebachiensis (M36921)Psammechinus miliaris (V01143/X01345) Litechinus pictus (X00628) Psammechinus lividus (M36920) |
Oligo (Nucl.Acids Res. 17:8543-51) ClustalX (Nucl.Acids Res. 24:4876-82) GeneDoc 2.3.000 (Nicholas&Nicholas, 1997) |
|
H4 |
Holothuria tubulosa (Z46226) |
Oligo (Nucl.Acids Res. 17:8543-51) |
|
Spacer |
Mytilus edulis (AJ224069) |
Mytilus edulis (AJ224069) |
|
Gene |
Coding Region Size (bp) |
% of Total Coding Region Amplified |
Efficiency |
|
H1 |
576 |
75 |
High |
|
H2A |
375 |
91 |
Low |
|
H2B |
372 |
69 |
Medium |
|
H3 |
411 |
77 |
High |
|
H4 |
312 |
79 |
High |
|
Spacer |
- |
unknown |
- |
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